Cellvax offers a global set of services:
- Anti-proliferative tests in vivo
- Tumoral invasion tests in vitro
- Tumoral invasion tests in vivo
- Original drug-resistant tumor models in vitro and in vivo
- Primary tumor cells
- Ascite production in mice
- Histological analysis
- Anti-virus tests in vitro and in vivo
- CNS tests in vitro and in vivo
Anti-proliferative tests in vivo
Cellvax proposes in their services to realize anti-proliferative tests in vitro on established cell lines or primary cultures. This technique allows at first to evaluate the anti-tumoral potential of drug candidates. Our company has also an important expertise in a three-dimensional cell culture method of cancer cells called nodules which has the advantage of maintaining some of the in vivo cellular architecture and interactions.
Tumoral invasion tests in vitro
Metastasis formation is the most deleterious mechanism in the tumour formation. Cellvax proposes in vitro tests which allow to measure the anti-metastatic potential of drug candidates. These techniques allow to study the intravasation and extravasation potentials of tumour cells through endothelium. The intravasation of tumour cells through endothelium is measured by a co-culture technique. Tumour cells were cultured on a micropore membrane in the top part of the chamber and endothelial cells were cultured in the low part of the chamber. This technique consists on measuring the number of tumour cells which have migrated through the micropore membrane toward to endothelial cells. The extravasation technique consists on measuring under flow tumour cells adhesion on endothelial cells.
Tumoral invasion tests in vivo
Cellvax proposes analyses of tumour cells distribution in all the animal. Stained tumour cells can be detected, counted and located in alive animal. This technique allows to determine the anti-metastatic potential of drug candidates.
Original drug-resistant tumor models in vitro and in vivo
Intrinsic or acquired tumor-mediated drug resistance is a major clinical problem that can result in the lack of tumor response to chemotherapy. In order to continuously improve the quality and the originality of Cellvax’s oncology services, we have successfully established several drug-resistant tumor models.
Primary tumor cells
Testing your promising compounds by using human primary tumor cells is the closest way to the real clinical situation. These data would be very useful for your future clinical trials. The established tumor cell lines, after numerous passages in vitro, might loose their initial biological characteristics. In contrast, these primary tumor cells (freshly established from human biopsies) conserve these biological characteristics and could be an ideal tool in anti-cancer studies.
Ascite production in mice
By administration of the incomplete Freund’s adjuvant.
- Tissues fixation in 4% Formalin;
- Frozen tissues;
- Paraffin-embedded or frozen sections;
- H & E and/or immuno-staining.
Anti-virus tests in vitro and in vivo
Infections with herpes simplex virus (HSV) types-1 and -2 are widespread in all human populations and result in persistent and latent infections. Cellvax proposes an entire validation test from in vitro to in vivo by using HSV-1, HSV-2 and HSV-R (ACV-resistant virus strain).
CNS tests in vitro and in vivo
An original test in vitro can allow to quickly find out if your innovating drugs have the capacity of going through the blood brain barrier (BBB) to reach the brain. If the obtained results are promising, an in vivo model in mice or rats can be proposed to further confirm its capacity to bypass the BBB.